Journal: International Journal of Oncology

Article Title: Synergistic effects of the combined treatment of U251 and T98G glioma cells with an anti-tubulin tetrahydrothieno[2,3-c]pyridine derivative and a peptide nucleic acid targeting miR-221-3p

doi: 10.3892/ijo.2021.5241

Figure Lengend Snippet: (A) Effects of (A) tetrahydrothieno[2,3-c]pyridine 3b and (B) R8-PNA-a221 on the growth of human glioma U251 cells. A summary of three independent experiments is presented. The data represent the mean ± SD. * P<0.05, ** P<0.01. PNA, peptide nucleic acid.

Article Snippet: The human glioma U251 ( ) and T98G ( ) cells were employed in the present study; both cell lines were purchased from Sigma-Aldrich; Merck KGaA.

Techniques:

Journal: International Journal of Oncology

Article Title: Synergistic effects of the combined treatment of U251 and T98G glioma cells with an anti-tubulin tetrahydrothieno[2,3-c]pyridine derivative and a peptide nucleic acid targeting miR-221-3p

doi: 10.3892/ijo.2021.5241

Figure Lengend Snippet: Effects of the tetrahydrothieno[2,3-c]pyridine 3b, R8-PNA-a221 and mutated R8-PNA-a221-MUT on the miR-221 content in human glioma U251 cells. Cells were cultured as indicated for 72 h, RNA was extracted and miR-221 quantified. A summary of three independent experiments is presented. The data represent the mean ± SD. ** P<0.01. PNA, peptide nucleic acid.

Article Snippet: The human glioma U251 ( ) and T98G ( ) cells were employed in the present study; both cell lines were purchased from Sigma-Aldrich; Merck KGaA.

Techniques: Cell Culture

Journal: International Journal of Oncology

Article Title: Synergistic effects of the combined treatment of U251 and T98G glioma cells with an anti-tubulin tetrahydrothieno[2,3-c]pyridine derivative and a peptide nucleic acid targeting miR-221-3p

doi: 10.3892/ijo.2021.5241

Figure Lengend Snippet: Effects of tetrahydrothieno[2,3-c]pyridine 3b and PNA-a221 on cell growth and viability. U251 cells were treated with the indicated concentrations of compound 3b and R8-PNA-a221 and (A) cell growth and cytotoxicity were determined. The anti-proliferative effects were assayed by employing a cell counter; cytotoxicity was evaluated by MTT assay. (B) Representative image of the clonogenic assay performed on U251 cells, and a summary of three independent experiments. Cells were fixed and stained with crystal violet after 10 days of treatment. (C) Morphological alteration of the treated U251 cells. Cells were treated as indicated and the morphological phenotype of the cells was analyzed by microphotographic analysis [magnification, ×100; combined treatment magnification, ×200 (left panel) and ×400 (right panel)]. PNA, peptide nucleic acid. * P<0.05, ** P<0.01, vs. untreated cells.

Article Snippet: The human glioma U251 ( ) and T98G ( ) cells were employed in the present study; both cell lines were purchased from Sigma-Aldrich; Merck KGaA.

Techniques: MTT Assay, Clonogenic Assay, Staining

Journal: International Journal of Oncology

Article Title: Synergistic effects of the combined treatment of U251 and T98G glioma cells with an anti-tubulin tetrahydrothieno[2,3-c]pyridine derivative and a peptide nucleic acid targeting miR-221-3p

doi: 10.3892/ijo.2021.5241

Figure Lengend Snippet: Effects of the tetrahydrothieno[2,3-c]pyridine 3b and PNA-a221 on the cell cycle and on the proportion of the sub-G1 population. U251 cells were treated with the indicated concentrations of compound 3b and R8-PNA-a221 and the cell cycle distribution was determined. (A) Representative cell cycle analyses. (B) Proportion of U251 cells in the G1, S and G2/M phase of the cell cycle (sub-G1 cells excluded from the calculations). (C) Increase in the sub-G1 cell population following treatment of the U251 cells with compound 3b, PNA-a221 or both 3b and PNA-a221 used in combination. PNA, peptide nucleic acid. * P<0.05, ** P<0.01, vs. untreated cells.

Article Snippet: The human glioma U251 ( ) and T98G ( ) cells were employed in the present study; both cell lines were purchased from Sigma-Aldrich; Merck KGaA.

Techniques:

Journal: International Journal of Oncology

Article Title: Synergistic effects of the combined treatment of U251 and T98G glioma cells with an anti-tubulin tetrahydrothieno[2,3-c]pyridine derivative and a peptide nucleic acid targeting miR-221-3p

doi: 10.3892/ijo.2021.5241

Figure Lengend Snippet: Effects of the tetrahydrothieno[2,3-c]pyridine 3b and PNA-a221 on the apoptosis of U251 cells, part I. U251 cells were treated with the indicated concentrations of compound 3b and R8-PNA-a221 and apoptosis was analyzed by Annexin V assay. Representative apoptosis analyses are reported. A summary of three independent experiments is presented in . PNA, peptide nucleic acid.

Article Snippet: The human glioma U251 ( ) and T98G ( ) cells were employed in the present study; both cell lines were purchased from Sigma-Aldrich; Merck KGaA.

Techniques: Annexin V Assay

Journal: International Journal of Oncology

Article Title: Synergistic effects of the combined treatment of U251 and T98G glioma cells with an anti-tubulin tetrahydrothieno[2,3-c]pyridine derivative and a peptide nucleic acid targeting miR-221-3p

doi: 10.3892/ijo.2021.5241

Figure Lengend Snippet: Effects of the tetrahydrothieno[2,3-c]pyridine 3b and PNA-a221 on the apoptosis of U251 cells, part II. U251 cells were treated with the indicated concentrations of compound 3b and R8-PNA-a221 and apoptosis was analyzed by caspase-3/7 assay. Representative apoptosis analyses are reported. A summary of three independent experiments is shown in . PNA, peptide nucleic acid.

Article Snippet: The human glioma U251 ( ) and T98G ( ) cells were employed in the present study; both cell lines were purchased from Sigma-Aldrich; Merck KGaA.

Techniques:

Journal: International Journal of Oncology

Article Title: Synergistic effects of the combined treatment of U251 and T98G glioma cells with an anti-tubulin tetrahydrothieno[2,3-c]pyridine derivative and a peptide nucleic acid targeting miR-221-3p

doi: 10.3892/ijo.2021.5241

Figure Lengend Snippet: Summary of three independent experiments analyzing the effects of the tetrahydrothieno[2,3-c]pyridine 3b and PNA-a221 on apoptosis, determined by (A) Annexin V and (B) caspase-3/7 assays. U251 cells were treated with the indicated concentrations of compound 3b and R8-PNA-a221 and apoptosis was analyzed. Representative data are shown in and . The dotted line represents the sum of the effects obtained using a single administration of compound 3b and R8-PNA-a221 at the lower concentration. PNA, peptide nucleic acid. * P<0.05, ** P<0.01.

Article Snippet: The human glioma U251 ( ) and T98G ( ) cells were employed in the present study; both cell lines were purchased from Sigma-Aldrich; Merck KGaA.

Techniques: Concentration Assay